In M.U.G.E.N, Dio has been made by various authors in both his original appearance from Phantom Blood and his appearance in Stardust Crusaders after assimilating Jonathan's body and acquiring a Stand, with most of these versions using his sprites from JoJo's Bizarre Adventure: Heritage for the Future. DIO's Stand, The World, is a close range stand that takes on the form of a muscular humanoid and is capable of extreme precision and brute strength The World's most notable ability, however, is its ability to temporarily stop time, during which only DIO and The World can move, thus rendering any opponent completely defenseless. After receiving Hamon training, Jonathan nearly kills Dio, who is forced to decapitate himself in order to survive.ДЎ00 years after his initial defeat, DIO emerges from a casket unearthed from the bottom of the sea, now having assimilated Jonathan's body and acquiring the ability to use a Stand this however, causes a chain reaction in the surviving members of the Joestar family as their Stand powers awaken as well. After Jonathan foiled Dio's plan, Dio threw away his humanity by killing George and using his blood to activate the Stone Mask, turning Dio into a vampire. After killing his father and learning of a debt his father owed to George Joestar I, Dio went to the Joestars with the intention of stealing their fortune for himself by tricking George into adopting him and secretly killing both George and his only son, Jonathan Joestar. Originally poor on the streets of Victorian England, Dio was the son of the thief Dario Brando, whom Dio held in contempt due to his abusive and alcoholic nature, holding him responsible for his mother's death. He is the main antagonist of both JoJo's Bizarre Adventure: Phantom Blood and JoJo's Bizarre Adventure: Stardust Crusaders.

There are versions of this character available that don't have their own branch articles!ДЄrtwork of Dio's Phantom Blood appearance fromДio Brando, later known as simply DIO, is a major antagonist from JoJo's Bizarre Adventure.

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